Publications

2024

Chemical genetic approaches to dissect microbiota mechanisms in health and disease.

Yang X, Hang HC. Science. 2024 Nov 15;386(6723):eado8548. doi: 10.1126/science.ado8548. Epub 2024 Nov 15.PMID: 39541443

Secreted antigen A peptidoglycan hydrolase is essential for Enterococcus faecium cell separation and priming of immune checkpoint inhibitor therapy.

Klupt S, Fam KT, Zhang X, Chodisetti PK, Mehmood A, Boyd T, Grotjahn D, Park D, Hang HC. Elife. 2024 Jun 10;13:RP95297. doi: 10.7554/eLife.95297.PMID: 38857064

Melanoma and microbiota: Current understanding and future directions.

Routy B, Jackson T, Mählmann L, Baumgartner CK, Blaser M, Byrd A, Corvaia N, Couts K, Davar D, Derosa L, Hang HC, Hospers G, Isaksen M, Kroemer G, Malard F, McCoy KD, Meisel M, Pal S, Ronai Z, Segal E, Sepich-Poore GD, Shaikh F, Sweis RF, Trinchieri G, van den Brink M, Weersma RK, Whiteson K, Zhao L, McQuade J, Zarour H, Zitvogel L. Cancer Cell. 2024 Jan 8;42(1):16-34. doi: 10.1016/j.ccell.2023.12.003. Epub 2023 Dec 28.PMID: 38157864

2023

Small molecule modulators of immune pattern recognition receptors.

Tsukidate T, Hespen CW, Hang HC. RSC Chem Biol. 2023 Oct 23;4(12):1014-1036. doi: 10.1039/d3cb00096f. eCollection 2023 Nov 29.PMID: 38033733

Discovery and Characterization of IFITM S-Palmitoylation.

Das T, Hang HC. Viruses. 2023 Nov 28;15(12):2329. doi: 10.3390/v15122329.PMID: 38140570

Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization.

Jang KK, Heaney T, London M, Ding Y, Putzel G, Yeung F, Ercelen D, Chen YH, Axelrad J, Gurunathan S, Zhou C, Podkowik M, Arguelles N, Srivastava A, Shopsin B, Torres VJ, Keestra-Gounder AM, Pironti A, Griffin ME, Hang HC, Cadwell K. Cell Host Microbe. 2023 Sep 13;31(9):1450-1468.e8. doi: 10.1016/j.chom.2023.08.002. Epub 2023 Aug 30.PMID: 37652008

N-Arylpyrazole NOD2 Agonists Promote Immune Checkpoint Inhibitor Therapy.

Griffin ME, Tsukidate T, Hang HC. ACS Chem Biol. 2023 Jun 16;18(6):1368-1377. doi: 10.1021/acschembio.3c00085. Epub 2023 May 12.PMID: 37172210

Chemoproteomics reveals microbiota-derived aromatic monoamine agonists for GPRC5A.

Zhao X, Stein KR, Chen V, Griffin ME, Lairson LL, Hang HC. Nat Chem Biol. 2023 Oct;19(10):1205-1214. doi: 10.1038/s41589-023-01328-z. Epub 2023 May 29.PMID: 37248411

Peptidoglycan NlpC/P60 peptidases in bacterial physiology and host interactions.

Griffin ME, Klupt S, Espinosa J, Hang HC. Cell Chem Biol. 2023 May 18;30(5):436-456. doi: 10.1016/j.chembiol.2022.11.001. Epub 2022 Nov 22.PMID: 36417916

Lytic bacteriophages facilitate antibiotic sensitization of Enterococcus faecium.

Canfield GS, Chatterjee A, Espinosa J, Mangalea MR, Sheriff EK, Keidan M, McBride SW, McCollister BD, Hang HC, Duerkop BA. Antimicrob Agents Chemother. 2023 May 1;65(5):e00143-21. doi: 10.1128/AAC.00143-21. Epub 2021 Mar 1.PMID: 33649110

Functional analysis of protein post-translational modifications using genetic codon expansion.

Peng T, Das T, Ding K, Hang HC. Protein Sci. 2023 Apr;32(4):e4618. doi: 10.1002/pro.4618.PMID: 36883310

Anti-infective bile acids bind and inactivate a Salmonella virulence regulator.

Yang X, Stein KR, Hang HC. Nat Chem Biol. 2023 Jan;19(1):91-100. doi: 10.1038/s41589-022-01122-3. Epub 2022 Sep 29.PMID: 36175659

2022

Chemoproteomic Analysis of Microbiota Metabolite-Protein Targets and Mechanisms.

Biochemistry. 2022 Dec 20;61(24):2822-2834. doi: 10.1021/acs.biochem.1c00758. Epub 2022 Jan 6.PMID: 34989554

Chemical proteomic analysis of bile acid-protein targets in Enterococcus faecium.

Yang X, Zhao X, Chen V, Hang HC. RSC Chem Biol. 2022 Sep 20;3(12):1397-1402. doi: 10.1039/d2cb00178k. eCollection 2022 Nov 30.PMID: 36544573

Identification of a Bile Acid-Binding Transcription Factor in Clostridioides difficile Using Chemical Proteomics.

Forster ER, Yang X, Tai AK, Hang HC, Shen A. ACS Chem Biol. 2022 Nov 18;17(11):3086-3099. doi: 10.1021/acschembio.2c00463. Epub 2022 Oct 24.PMID: 36279369

Microbial mechanisms to improve immune checkpoint blockade responsiveness.

Griffin ME, Hang HC. Neoplasia. 2022 Sep;31:100818. doi: 10.1016/j.neo.2022.100818. Epub 2022 Jul 8.PMID: 35816968

S-Palmitoylation and Sterol Interactions Mediate Antiviral Specificity of IFITMs.

Das T, Yang X, Lee H, Garst EH, Valencia E, Chandran K, Im W, Hang HC. ACS Chem Biol. 2022 Aug 19;17(8):2109-2120. doi: 10.1021/acschembio.2c00176. Epub 2022 Jul 21.PMID: 35861660

Membrane targeting enhances muramyl dipeptide binding to NOD2 and Arf6-GTPase in mammalian cells. Hespen CW, Zhao X, Hang HC. Chem Commun (Camb). 2022 Jun 8;58(46):6598-6601. doi: 10.1039/d2cc01903e. PMID: 35584401

Improving immunotherapy response through the use of designer bacteria.

Griffin ME, Hang HC. Cancer Cell. 2021 Dec 13;39(12):1576-1577. doi: 10.1016/j.ccell.2021.11.009.PMID: 34906317

Chemical approaches for investigating site-specific protein S-fatty acylation.

Garst EH, Das T, Hang HC. Curr Opin Chem Biol. 2021 Dec;65:109-117. doi: 10.1016/j.cbpa.2021.06.004. Epub 2021 Jul 29.PMID: 34333222

2021

Improving immunotherapy response through the use of designer bacteria.

Griffin ME, Hang HC. Cancer Cell. 2021 Dec 13;39(12):1576-1577. doi: 10.1016/j.ccell.2021.11.009. PMID: 34906317

Chemical approaches for investigating site-specific protein S-fatty acylation.

Garst EH, Das T, Hang HC. Curr Opin Chem Biol. 2021 Dec;65:109-117. doi: 10.1016/j.cbpa.2021.06.004. Epub 2021 Jul 29.PMID: 34333222.

Enterococcus peptidoglycan remodeling promotes checkpoint inhibitor cancer immunotherapy.

Griffin ME, Espinosa J, Becker JL, Luo JD, Carroll TS, Jha JK, Fanger GR, Hang HC. Science. 2021 Aug 27;373(6558):1040-1046. doi: 10.1126/science.abc9113.PMID: 34446607

• Perspective: How microbiota improve immunotherapy. Ansaldo E, Belkaid Y. Science. 2021 Aug 27;373(6558):966-967. doi: 10.1126/science.abl3656.PMID: 34446595

RecT recombinase expression enables efficient gene editing in Enterococcus.

Chen V, Griffin ME, Maguin P, Varble A, Hang HC. Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 10.1128/AEM.00844-21. Online ahead of print.PMID: 34232061

Chemical approaches for investigating site-specific protein S-fatty acylation.

Garst EH, Das T, Hang HC. Curr Opin Chem Biol. 2021 Jul 29;65:109-117. doi: 10.1016/j.cbpa.2021.06.004. PMID: 34333222

Site-Specific Lipidation Enhances IFITM3 Membrane Interactions and Antiviral Activity.

Garst EH, Lee H, Das T, Bhattacharya S, Percher A, Wiewiora R, Witte IP, Li Y, Peng T, Im W, Hang HC. ACS Chem Biol. 2021 May 21;16(5):844-856. doi: 10.1021/acschembio.1c00013. Epub 2021 Apr 22.PMID: 33887136

Protein S-palmitoylation in immunity.

Das T, Yount JS, Hang HC. Open Biol. 2021 Mar;11(3):200411. doi: 10.1098/rsob.200411. Epub 2021 Mar 3.PMID: 33653086

2020

Enterococcus NlpC/p60 Peptidoglycan Hydrolase SagA Localizes to Sites of Cell Division and Requires Only a Catalytic Dyad for Protease Activity.

Espinosa J, Lin TY, Estrella Y, Kim B, Molina H, Hang HC. Biochemistry. 2020 Nov 24;59(46):4470-4480. doi: 10.1021/acs.biochem.0c00755. Epub 2020 Nov 2.PMID: 33136372

Site-specific chemical fatty-acylation for gain-of-function analysis of protein S-palmitoylation in live cells.

Li Y , Wang S , Chen Y , Li M , Dong X , Hang HC , Peng T . Chem Commun (Camb). 2020 Nov 18;56(89):13880-13883. doi: 10.1039/d0cc06073a. Epub 2020 Oct 23.PMID: 33094750

Nuclear Receptor Chemical Reporter Enables Domain-Specific Analysis of Ligands in Mammalian Cells.

Tsukidate T, Li Q, Hang HC. ACS Chem Biol. 2020 Sep 18;15(9):2324-2330. doi: 10.1021/acschembio.0c00432. Epub 2020 Sep 10.PMID: 32909738

Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.

Wu X, Spence JS, Das T, Yuan X, Chen C, Zhang Y, Li Y, Sun Y, Chandran K, Hang HC, Peng T. Cell Chem Biol. 2020 May 21;27(5):571-585.e6. doi: 10.1016/j.chembiol.2020.03.004. Epub 2020 Apr 2.PMID: 32243810

Translation of Microbiota Short-Chain Fatty Acid Mechanisms Affords Anti-infective Acyl-Salicylic Acid Derivatives.

Yang X, Forster ER, Darabedian N, Kim AT, Pratt MR, Shen A, Hang HC. ACS Chem Biol. 2020 May 15;15(5):1141-1147. doi: 10.1021/acschembio.9b01009. Epub 2020 Mar 2.PMID: 32091869

Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity.

Kim B, Espinosa J, Hang HC. Methods Enzymol. 2020;638:109-127. doi: 10.1016/bs.mie.2020.02.017. Epub 2020 Mar 31.PMID: 32416909

Chemical Reporters for Exploring Microbiology and Microbiota Mechanisms.

Zhang ZJ, Wang YC, Yang X, Hang HC. Chembiochem. 2020 Jan 15;21(1-2):19-32. doi: 10.1002/cbic.201900535. Epub 2019 Dec 27.PMID: 31730246

Translation of peptidoglycan metabolites into immunotherapeutics.

Griffin ME, Hespen CW, Wang YC, Hang HC. Clin Transl Immunology. 2019 Nov 29;8(12):e1095. doi: 10.1002/cti2.1095. eCollection 2019.PMID: 31798878

Targeted and proteome-wide analysis of metabolite-protein interactions.

Tsukidate T, Li Q, Hang HC. Curr Opin Chem Biol. 2020 Feb;54:19-27. doi: 10.1016/j.cbpa.2019.10.008. Epub 2019 Nov 29.PMID: 31790852

Site-specific acylation of a bacterial virulence regulator attenuates infection.

Zhang ZJ, Pedicord VA, Peng T, Hang HC. Nat Chem Biol. 2020 Jan;16(1):95-103. doi: 10.1038/s41589-019-0392-5. Epub 2019 Nov 18.PMID: 31740807

2019

Enterococcus faecium secreted antigen A generates muropeptides to enhance host immunity and limit bacterial pathogenesis.

Kim B, Wang YC, Hespen CW, Espinosa J, Salje J, Rangan KJ, Oren DA, Kang JY, Pedicord VA, Hang HC. Elife. 2019 Apr 10;8:e45343. doi: 10.7554/eLife.45343.PMID: 30969170

CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4.

Zhu XG, Nicholson Puthenveedu S, Shen Y, La K, Ozlu C, Wang T, Klompstra D, Gultekin Y, Chi J, Fidelin J, Peng T, Molina H, Hang HC, Min W, Birsoy K. Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi: 10.1016/j.molcel.2019.01.037. Epub 2019 Mar 4.PMID: 30846317

Peptidoglycan Metabolite Photoaffinity Reporters Reveal Direct Binding to Intracellular Pattern Recognition Receptors and Arf GTPases.

Wang YC, Westcott NP, Griffin ME, Hang HC.ACS Chem Biol. 2019 Mar 15;14(3):405-414. doi: 10.1021/acschembio.8b01038. Epub 2019 Feb 20.PMID: 30735346

IFITM3 directly engages and shuttles incoming virus particles to lysosomes.

Spence JS, He R, Hoffmann HH, Das T, Thinon E, Rice CM, Peng T, Chandran K, Hang HC. Nat Chem Biol. 2019 Mar;15(3):259-268. doi: 10.1038/s41589-018-0213-2. Epub 2019 Jan 14.PMID: 30643282

Chemical Proteomic Analysis of S-Fatty Acylated Proteins and Their Modification Sites.

Thinon E, Hang HC. Methods Mol Biol. 2019;2009:45-57. doi: 10.1007/978-1-4939-9532-5_4.PMID: 31152394

Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens.

Peng T, Hang HC. Curr Top Microbiol Immunol. 2019;420:93-110. doi: 10.1007/82_2018_126.PMID: 30128826

2018

Nε-fatty acylation of multiple membrane-associated proteins by Shigella IcsB effector to modulate host function.

Liu W, Zhou Y, Peng T, Zhou P, Ding X, Li Z, Zhong H, Xu Y, Chen S, Hang HC, Shao F. Nat Microbiol. 2018 Sep;3(9):996-1009. doi: 10.1038/s41564-018-0215-6. Epub 2018 Jul 30.PMID: 30061757

Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites.

Thinon E, Fernandez JP, Molina H, Hang HC. J Proteome Res. 2018 May 4;17(5):1907-1922. doi: 10.1021/acs.jproteome.8b00002. Epub 2018 Apr 6.PMID: 29575903

2017

The palmitoyltransferase ZDHHC20 enhances interferon-induced transmembrane protein 3 (IFITM3) palmitoylation and antiviral activity.

McMichael TM, Zhang L, Chemudupati M, Hach JC, Kenney AD, Hang HC, Yount JS. J Biol Chem. 2017 Dec 29;292(52):21517-21526. doi: 10.1074/jbc.M117.800482. Epub 2017 Oct 27.PMID: 29079573

Biochemical Mechanisms of Pathogen Restriction by Intestinal Bacteria.

Rangan KJ, Hang HC. Trends Biochem Sci. 2017 Nov;42(11):887-898. doi: 10.1016/j.tibs.2017.08.005. Epub 2017 Sep 17.PMID: 28927699

Epigallocatechin-3-gallate inhibits bacterial virulence and invasion of host cells.

Tsou LK, Yount JS, Hang HC. Bioorg Med Chem. 2017 Jun 1;25(11):2883-2887. doi: 10.1016/j.bmc.2017.03.023. Epub 2017 Mar 10.PMID: 28325635

Protein S-palmitoylation in cellular differentiation.

Zhang MM, Hang HC. Biochem Soc Trans. 2017 Feb 8;45(1):275-285. doi: 10.1042/BST20160236.PMID: 28202682

Chemical proteomics reveals ADP-ribosylation of small GTPases during oxidative stress.

Westcott NP, Fernandez JP, Molina H, Hang HC. Nat Chem Biol. 2017 Mar;13(3):302-308. doi: 10.1038/nchembio.2280. Epub 2017 Jan 16.PMID: 28092360

2016

Site-Specific Bioorthogonal Labeling for Fluorescence Imaging of Intracellular Proteins in Living Cells.

Peng T, Hang HC. J Am Chem Soc. 2016 Nov 2;138(43):14423-14433. doi: 10.1021/jacs.6b08733. Epub 2016 Oct 21.PMID: 27768298

A secreted bacterial peptidoglycan hydrolase enhances tolerance to enteric pathogens.

Rangan KJ, Pedicord VA, Wang YC, Kim B, Lu Y, Shaham S, Mucida D, Hang HC. Science. 2016 Sep 23;353(6306):1434-1437. doi: 10.1126/science.aaf3552. Epub 2016 Sep 22.PMID: 27708039

Exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance.

Pedicord VA, Lockhart AAK, Rangan KJ, Craig JW, Loschko J, Rogoz A, Hang HC, Mucida D. Sci Immunol. 2016 Sep;1(3):eaai7732. doi: 10.1126/sciimmunol.aai7732. Epub 2016 Sep 22.PMID: 28580440

Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.

Thinon E, Percher A, Hang HC. Chembiochem. 2016 Oct 4;17(19):1800-1803. doi: 10.1002/cbic.201600213. Epub 2016 Jul 29.PMID: 27350074

Mass-tag labeling reveals site-specific and endogenous levels of protein S-fatty acylation.

Percher A, Ramakrishnan S, Thinon E, Yuan X, Yount JS, Hang HC. Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4302-7. doi: 10.1073/pnas.1602244113. Epub 2016 Apr 4.PMID: 27044110

Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion Substrates.

Tsou LK, Lara-Tejero M, RoseFigura J, Zhang ZJ, Wang YC, Yount JS, Lefebre M, Dossa PD, Kato J, Guan F, Lam W, Cheng YC, Galán JE, Hang HC. J Am Chem Soc. 2016 Feb 24;138(7):2209-18. doi: 10.1021/jacs.5b11575. Epub 2016 Feb 16.PMID: 26847396

Proteomic analysis of fatty-acylated proteins.

Peng T, Thinon E, Hang HC. Curr Opin Chem Biol. 2016 Feb;30:77-86. doi: 10.1016/j.cbpa.2015.11.008. Epub 2015 Dec 2.PMID: 26656971

2015

A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.

Santiago-Tirado FH, Peng T, Yang M, Hang HC, Doering TL. PLoS Pathog. 2015 May 13;11(5):e1004908. doi: 10.1371/journal.ppat.1004908. eCollection 2015 May.PMID: 25970403

Chemical reporters for exploring protein acylation.

Thinon E, Hang HC. Biochem Soc Trans. 2015 Apr;43(2):253-61. doi: 10.1042/BST20150004.PMID: 25849926

Myristoylome profiling reveals a concerted mechanism of ARF GTPase deacylation by the bacterial protease IpaJ.

Burnaevskiy N, Peng T, Reddick LE, Hang HC, Alto NM. Mol Cell. 2015 Apr 2;58(1):110-22. doi: 10.1016/j.molcel.2015.01.040. Epub 2015 Mar 12.PMID: 25773595

Bifunctional fatty acid chemical reporter for analyzing S-palmitoylated membrane protein-protein interactions in mammalian cells.

Peng T, Hang HC. J Am Chem Soc. 2015 Jan 21;137(2):556-9. doi: 10.1021/ja502109n. Epub 2015 Jan 12.PMID: 25575299

Integrative chemical biology approaches for identification and characterization of "erasers" for fatty-acid-acylated lysine residues within proteins.

Liu Z, Yang T, Li X, Peng T, Hang HC, Li XD. Angew Chem Int Ed Engl. 2015 Jan 19;54(4):1149-52. doi: 10.1002/anie.201408763. Epub 2014 Dec 4.PMID: 25476551

2014

Chemical reporters for exploring ADP-ribosylation and AMPylation at the host-pathogen interface.

Westcott NP, Hang HC. Curr Opin Chem Biol. 2014 Dec;23:56-62. doi: 10.1016/j.cbpa.2014.10.002.PMID: 25461386

Chemoproteomics reveals Toll-like receptor fatty acylation.

Chesarino NM, Hach JC, Chen JL, Zaro BW, Rajaram MV, Turner J, Schlesinger LS, Pratt MR, Hang HC, Yount JS. BMC Biol. 2014 Nov 5;12:91. doi: 10.1186/s12915-014-0091-3.PMID: 25371237

Turning the spotlight on protein-lipid interactions in cells.

Peng T, Yuan X, Hang HC. Curr Opin Chem Biol. 2014 Aug;21:144-53. doi: 10.1016/j.cbpa.2014.07.015. Epub 2014 Aug 15.PMID: 25129056

Copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host AMPylation on self-assembled human protein microarrays.

Yu X, Woolery AR, Luong P, Hao YH, Grammel M, Westcott N, Park J, Wang J, Bian X, Demirkan G, Hang HC, Orth K, LaBaer J. Mol Cell Proteomics. 2014 Nov;13(11):3164-76. doi: 10.1074/mcp.M114.041103. Epub 2014 Jul 29.PMID: 25073739

SORTing out cellular proteomes in vivo.

Peng T, Hang HC. Nat Biotechnol. 2014 May;32(5):445-6. doi: 10.1038/nbt.2898.PMID: 24811517

2013

Chemical reporters for biological discovery.

Grammel M, Hang HC. Nat Chem Biol. 2013 Aug;9(8):475-84. doi: 10.1038/nchembio.1296.PMID: 23868317

Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast.

Zhang MM, Wu PY, Kelly FD, Nurse P, Hang HC. PLoS Biol. 2013 Jul;11(7):e1001597. doi: 10.1371/journal.pbio.1001597. Epub 2013 Jul 2.PMID: 23843742

Prenylome profiling reveals S-farnesylation is crucial for membrane targeting and antiviral activity of ZAP long-isoform.

Charron G, Li MM, MacDonald MR, Hang HC. Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11085-90. doi: 10.1073/pnas.1302564110. Epub 2013 Jun 17.PMID: 23776219

SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine.

Jiang H, Khan S, Wang Y, Charron G, He B, Sebastian C, Du J, Kim R, Ge E, Mostoslavsky R, Hang HC, Hao Q, Lin H. Nature. 2013 Apr 4;496(7443):110-3. doi: 10.1038/nature12038.PMID: 23552949

Emerging roles for protein S-palmitoylation in immunity from chemical proteomics.

Yount JS, Zhang MM, Hang HC. Curr Opin Chem Biol. 2013 Feb;17(1):27-33. doi: 10.1016/j.cbpa.2012.11.008. Epub 2013 Jan 14.PMID: 23332315

Small molecules aimed at type III secretion systems to inhibit bacterial virulence.

Tsou LK, Dossa PD, Hang HC. Medchemcomm. 2013 Jan 1;4(1):68-79. doi: 10.1039/C2MD20213A.PMID: 23930198

Identification of lysine acetyltransferase substrates using bioorthogonal chemical proteomics.

Grammel M, Hang HC. Methods Mol Biol. 2013;981:201-10. doi: 10.1007/978-1-62703-305-3_16.PMID: 23381864

Incorporation of unnatural sugars for the identification of glycoproteins.

Zaro BW, Hang HC, Pratt MR. Methods Mol Biol. 2013;951:57-67. doi: 10.1007/978-1-62703-146-2_5.PMID: 23296524

2012

Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation.

Zhang X, Abreu JG, Yokota C, MacDonald BT, Singh S, Coburn KL, Cheong SM, Zhang MM, Ye QZ, Hang HC, Steen H, He X. Cell. 2012 Jun 22;149(7):1565-77. doi: 10.1016/j.cell.2012.04.039.PMID: 22726442

S-palmitoylation and ubiquitination differentially regulate interferon-induced transmembrane protein 3 (IFITM3)-mediated resistance to influenza virus.

Yount JS, Karssemeijer RA, Hang HC.J Biol Chem. 2012 Jun 1;287(23):19631-41. doi: 10.1074/jbc.M112.362095. Epub 2012 Apr 17.PMID: 22511783

Cell-selective labeling of bacterial proteomes with an orthogonal phenylalanine amino acid reporter.

Grammel M, Dossa PD, Taylor-Salmon E, Hang HC. Chem Commun (Camb). 2012 Feb 1;48(10):1473-4. doi: 10.1039/c1cc14939c. Epub 2011 Nov 11.PMID: 22080199

Bioorthogonal proteomics of 15-hexadecynyloxyacetic acid chemical reporter reveals preferential targeting of fatty acid modified proteins and biosynthetic enzymes.

Yount JS, Charron G, Hang HC. Bioorg Med Chem. 2012 Jan 15;20(2):650-4. doi: 10.1016/j.bmc.2011.03.062. Epub 2011 Apr 3.PMID: 21524915

2011

Palmitoylation of MICA, a ligand for NKG2D, mediates its recruitment to membrane microdomains and promotes its shedding.

Agüera-González S, Gross CC, Fernández-Messina L, Ashiru O, Esteso G, Hang HC, Reyburn HT, Long EO, Valés-Gómez M. Eur J Immunol. 2011 Dec;41(12):3667-76. doi: 10.1002/eji.201141645. Epub 2011 Nov 3.PMID: 21928280

A chemical reporter for protein AMPylation.

Grammel M, Luong P, Orth K, Hang HC. J Am Chem Soc. 2011 Nov 2;133(43):17103-5. doi: 10.1021/ja205137d. Epub 2011 Oct 7.PMID: 21942216

Exploring protein lipidation with chemical biology.

Hang HC, Linder ME. Chem Rev. 2011 Oct 12;111(10):6341-58. doi: 10.1021/cr2001977. Epub 2011 Sep 16.PMID: 21919527

Subcellular targeting of Salmonella virulence proteins by host-mediated S-palmitoylation.

Hicks SW, Charron G, Hang HC, Galán JE. Cell Host Microbe. 2011 Jul 21;10(1):9-20. doi: 10.1016/j.chom.2011.06.003.PMID: 21767808

Bioorthogonal chemical reporters for analyzing protein lipidation and lipid trafficking.

Hang HC, Wilson JP, Charron G.Acc Chem Res. 2011 Sep 20;44(9):699-708. doi: 10.1021/ar200063v. Epub 2011 Jun 15.PMID: 21675729

Identification of lysine acetyltransferase p300 substrates using 4-pentynoyl-coenzyme A and bioorthogonal proteomics.

Yang YY, Grammel M, Hang HC. Bioorg Med Chem Lett. 2011 Sep 1;21(17):4976-9. doi: 10.1016/j.bmcl.2011.05.060. Epub 2011 May 25.PMID: 21669532

Chemical reporters for fluorescent detection and identification of O-GlcNAc-modified proteins reveal glycosylation of the ubiquitin ligase NEDD4-1.

Zaro BW, Yang YY, Hang HC, Pratt MR. Proc Natl Acad Sci U S A. 2011 May 17;108(20):8146-51. doi: 10.1073/pnas.1102458108. Epub 2011 May 3.PMID: 21540332

Visualization and Identification of Fatty Acylated Proteins Using Chemical Reporters.

Yount JS, Zhang MM, Hang HC. Curr Protoc Chem Biol. 2011 May 1;3(2):65-79. doi: 10.1002/9780470559277.ch100225.PMID: 23061028

Differential regulation of two palmitoylation sites in the cytoplasmic tail of the beta1-adrenergic receptor.

Zuckerman DM, Hicks SW, Charron G, Hang HC, Machamer CE. J Biol Chem. 2011 May 27;286(21):19014-23. doi: 10.1074/jbc.M110.189977. Epub 2011 Apr 4.PMID: 21464135

Chemical approaches for the detection and synthesis of acetylated proteins.

Yang YY, Hang HC. Chembiochem. 2011 Jan 24;12(2):314-22. doi: 10.1002/cbic.201000558. Epub 2011 Jan 11.PMID: 21243719

Alkynyl-farnesol reporters for detection of protein S-prenylation in cells.

Charron G, Tsou LK, Maguire W, Yount JS, Hang HC. Mol Biosyst. 2011 Jan;7(1):67-73. doi: 10.1039/c0mb00183j. Epub 2010 Nov 25.PMID: 21107478

Proteomic analysis of fatty-acylated proteins in mammalian cells with chemical reporters reveals S-acylation of histone H3 variants.

Wilson JP, Raghavan AS, Yang YY, Charron G, Hang HC. Mol Cell Proteomics. 2011 Mar;10(3):M110.001198. doi: 10.1074/mcp.M110.001198. Epub 2010 Nov 14.PMID: 21076176

2010

Comparative analysis of cleavable azobenzene-based affinity tags for bioorthogonal chemical proteomics.

Yang YY, Grammel M, Raghavan AS, Charron G, Hang HC. Chem Biol. 2010 Nov 24;17(11):1212-22. doi: 10.1016/j.chembiol.2010.09.012.PMID: 21095571

Lipidation by the host prenyltransferase machinery facilitates membrane localization of Legionella pneumophila effector proteins.

Ivanov SS, Charron G, Hang HC, Roy CR. J Biol Chem. 2010 Nov 5;285(45):34686-98. doi: 10.1074/jbc.M110.170746. Epub 2010 Sep 2.PMID: 20813839

Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters.

Rangan KJ, Yang YY, Charron G, Hang HC. J Am Chem Soc. 2010 Aug 11;132(31):10628-9. doi: 10.1021/ja101387b.PMID: 20230003

Orthogonal alkynyl amino acid reporter for selective labeling of bacterial proteomes during infection.

Grammel M, Zhang MM, Hang HC. Angew Chem Int Ed Engl. 2010 Aug 9;49(34):5970-4. doi: 10.1002/anie.201002050.PMID: 20632346

Palmitoylome profiling reveals S-palmitoylation-dependent antiviral activity of IFITM3.

Yount JS, Moltedo B, Yang YY, Charron G, Moran TM, López CB, Hang HC. Nat Chem Biol. 2010 Aug;6(8):610-4. doi: 10.1038/nchembio.405. Epub 2010 Jul 4.PMID: 20601941

Visible fluorescence detection of type III protein secretion from bacterial pathogens.

Yount JS, Tsou LK, Dossa PD, Kullas AL, van der Velden AW, Hang HC. J Am Chem Soc. 2010 Jun 23;132(24):8244-5. doi: 10.1021/ja102257v.PMID: 20504019

Tandem fluorescence imaging of dynamic S-acylation and protein turnover.

Zhang MM, Tsou LK, Charron G, Raghavan AS, Hang HC. Proc Natl Acad Sci U S A. 2010 May 11;107(19):8627-32. doi: 10.1073/pnas.0912306107. Epub 2010 Apr 26.PMID: 20421494

Bioorthogonal chemical reporters for monitoring protein acetylation.

Yang YY, Ascano JM, Hang HC. J Am Chem Soc. 2010 Mar 24;132(11):3640-1. doi: 10.1021/ja908871t.PMID: 20192265

2009

Clickable fluorescent dyes for multimodal bioorthogonal imaging.

Tsou LK, Zhang MM, Hang HC. Org Biomol Chem. 2009 Dec 21;7(24):5055-8. doi: 10.1039/b917119n. Epub 2009 Oct 26.PMID: 20024096

Chemical tools for understanding protein lipidation in eukaryotes.

Charron G, Wilson J, Hang HC. Curr Opin Chem Biol. 2009 Oct;13(4):382-91. doi: 10.1016/j.cbpa.2009.07.010. Epub 2009 Aug 19.PMID: 19699139

Robust fluorescent detection of protein fatty-acylation with chemical reporters.

Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC. J Am Chem Soc. 2009 Apr 8;131(13):4967-75. doi: 10.1021/ja810122f.PMID: 19281244

Seeing small molecules in action with bioorthogonal chemistry.

Raghavan AS, Hang HC. Drug Discov Today. 2009 Feb;14(3-4):178-84. doi: 10.1016/j.drudis.2008.09.014. Epub 2008 Nov 18.PMID: 18973827

2008

Chemical probes for profiling fatty acid-associated proteins in living cells.

Raghavan A, Charron G, Flexner J, Hang HC. Bioorg Med Chem Lett. 2008 Nov 15;18(22):5982-6. doi: 10.1016/j.bmcl.2008.09.083. Epub 2008 Sep 30.PMID: 18929483

Lipid-independent secretion of a Drosophila Wnt protein.

Ching W, Hang HC, Nusse R. J Biol Chem. 2008 Jun 20;283(25):17092-8. doi: 10.1074/jbc.M802059200. Epub 2008 Apr 22.PMID: 18430724

Hang Postdoctoral, Graduate and Undergraduate Publications

Lysosomal cysteine and aspartic proteases are heterogeneously expressed and act redundantly to initiate human invariant chain degradation.

Costantino CM, Hang HC, Kent SC, Hafler DA, Ploegh HL. J Immunol. 2008 Mar 1;180(5):2876-85. doi: 10.4049/jimmunol.180.5.2876.PMID: 18292509

Chemical probes for the rapid detection of Fatty-acylated proteins in Mammalian cells.

Hang HC, Geutjes EJ, Grotenbreg G, Pollington AM, Bijlmakers MJ, Ploegh HL. J Am Chem Soc. 2007 Mar 14;129(10):2744-5. doi: 10.1021/ja0685001. Epub 2007 Feb 17.PMID: 17305342

Mechanism-based probe for the analysis of cathepsin cysteine proteases in living cells.

Hang HC, Loureiro J, Spooner E, van der Velden AW, Kim YM, Pollington AM, Maehr R, Starnbach MN, Ploegh HL. ACS Chem Biol. 2006 Dec 20;1(11):713-23. doi: 10.1021/cb600431a.PMID: 17184136

The chemistry and biology of mucin-type O-linked glycosylation.

Hang HC, Bertozzi CR. Bioorg Med Chem. 2005 Sep 1;13(17):5021-34. doi: 10.1016/j.bmc.2005.04.085.PMID: 16005634

Asparagine endopeptidase is not essential for class II MHC antigen presentation but is required for processing of cathepsin L in mice.

Maehr R, Hang HC, Mintern JD, Kim YM, Cuvillier A, Nishimura M, Yamada K, Shirahama-Noda K, Hara-Nishimura I, Ploegh HL. J Immunol. 2005 Jun 1;174(11):7066-74. doi: 10.4049/jimmunol.174.11.7066.PMID: 15905550

Catching proteases in action with microarrays.

Hang HC, Ploegh H.Chem Biol. 2004 Oct;11(10):1328-30. doi: 10.1016/j.chembiol.2004.09.005.PMID: 15489158

Deconvoluting the functions of polypeptide N-alpha-acetylgalactosaminyltransferase family members by glycopeptide substrate profiling.

Pratt MR, Hang HC, Ten Hagen KG, Rarick J, Gerken TA, Tabak LA, Bertozzi CR.Chem Biol. 2004 Jul;11(7):1009-16. doi: 10.1016/j.chembiol.2004.05.009.PMID: 15271359

Small molecule inhibitors of mucin-type O-linked glycosylation from a uridine-based library.

Hang HC, Yu C, Ten Hagen KG, Tian E, Winans KA, Tabak LA, Bertozzi CR.Chem Biol. 2004 Mar;11(3):337-45. doi: 10.1016/j.chembiol.2004.02.023.PMID: 15123263

Probing glycosyltransferase activities with the Staudinger ligation.

Hang HC, Yu C, Pratt MR, Bertozzi CR.J Am Chem Soc. 2004 Jan 14;126(1):6-7. doi: 10.1021/ja037692m.PMID: 14709032

A metabolic labeling approach toward proteomic analysis of mucin-type O-linked glycosylation.

Hang HC, Yu C, Kato DL, Bertozzi CR.Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14846-51. doi: 10.1073/pnas.2335201100. Epub 2003 Dec 1.PMID: 14657396

cDNA cloning and expression of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase T1 from Toxoplasma gondii.

Wojczyk BS, Stwora-Wojczyk MM, Hagen FK, Striepen B, Hang HC, Bertozzi CR, Roos DS, Spitalnik SL.Mol Biochem Parasitol. 2003 Oct;131(2):93-107. doi: 10.1016/s0166-6851(03)00196-8.PMID: 14511808

A chemical approach for identifying O-GlcNAc-modified proteins in cells.

Vocadlo DJ, Hang HC, Kim EJ, Hanover JA, Bertozzi CR.Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9116-21. doi: 10.1073/pnas.1632821100. Epub 2003 Jul 21.PMID: 12874386

Constructing azide-labeled cell surfaces using polysaccharide biosynthetic pathways.

Luchansky SJ, Hang HC, Saxon E, Grunwell JR, Yu C, Dube DH, Bertozzi CR.Methods Enzymol. 2003;362:249-72. doi: 10.1016/S0076-6879(03)01018-8.PMID: 12968369

Investigating cellular metabolism of synthetic azidosugars with the Staudinger ligation.

Saxon E, Luchansky SJ, Hang HC, Yu C, Lee SC, Bertozzi CR.J Am Chem Soc. 2002 Dec 18;124(50):14893-902. doi: 10.1021/ja027748x.PMID: 12475330

Substrate specificity of the sialic acid biosynthetic pathway.

Jacobs CL, Goon S, Yarema KJ, Hinderlich S, Hang HC, Chai DH, Bertozzi CR.Biochemistry. 2001 Oct 30;40(43):12864-74. doi: 10.1021/bi010862s.PMID: 11669623

Chemoselective approaches to glycoprotein assembly.

Hang HC, Bertozzi CR.Acc Chem Res. 2001 Sep;34(9):727-36. doi: 10.1021/ar9901570.PMID: 11560472

Ketone isosteres of 2-N-acetamidosugars as substrates for metabolic cell surface engineering.

Hang HC, Bertozzi CR.J Am Chem Soc. 2001 Feb 14;123(6):1242-3. doi: 10.1021/ja002962b.PMID: 11456684

The Synthesis of 3-Methoxycarbonylbenzofuran-2(3H)-one Derivatives via Copper(I)-Catalyzed Coupling of o-Bromophenols with Dimethyl Malonate.

Hang HC, Drotleff E, Elliot GI, Ritsema TA, Konopelski JP. Synthesis. 1999;3:398-400.